James Anderson has been ruled out for the remainder of the Ashes as a calf injury continues to cause him problems.
Anderson managed just four overs in the first Test earlier this month before limping off.
There was some hope that he would be able to return to the England towards the end of the series but that will no longer be possible.
The injury to his right calf has plagued the 37-year-old for a number of weeks.
He stepped up his recovery by bowling 20 overs on Tuesday while representing Lancashire in a second-string fixture against Durham at Chester Boughton Hall and sent down a further nine on Thursday.
But it was at the conclusion of his nine-over spell where he became aware of more pain to his calf, and after consultation with the England medical team, he was made unavailable for the rest of the Ashes.
Anderson will therefore miss out at his home ground of Old Trafford for the Test which gets under way on September 4.
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Somerset’s Jamie Overton has been added to the 12 that was named ahead of last week’s dramatic one-wicket victory at Headingley.
Overton made his international debut in the 2017-18 Ashes series Down Under while he earned the last of his three Test caps against New Zealand in Auckland.
That was the Test where England succumbed to 58 all out, their sixth-lowest of all-time, although Overton’s unbeaten 33 saved them from an even more ignominious fate.
He has collected 32 wickets at an average of 21.34 in eight championship matches this year to put himself back into England contention.
There has been some scrutiny on Jason Roy, whose average from seven innings as a Test opener stands at 8.85, but he has been retained, meaning Surrey team-mate Ollie Pope – tipped for a recall – misses out.
England squad: Joe Root, Jofra Archer, Jonny Bairstow, Stuart Broad, Rory Burns, Jos Buttler, Sam Curran, Joe Denly, Jack Leach, Craig Overton, Jason Roy, Ben Stokes, Chris Woakes.